|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human SULT1E1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11275-ACG|
|Human SULT1E1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11275-ACR|
|Human SULT1E1 ORF mammalian expression plasmid, N-GFPSpark tag||HG11275-ANG|
|Human SULT1E1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11275-ANR|
|Human SULT1E1 ORF mammalian expression plasmid, C-Flag tag||HG11275-CF|
|Human SULT1E1 ORF mammalian expression plasmid, C-His tag||HG11275-CH|
|Human SULT1E1 ORF mammalian expression plasmid, C-Myc tag||HG11275-CM|
|Human SULT1E1 ORF mammalian expression plasmid, C-HA tag||HG11275-CY|
|Human SULT1E1 natural ORF mammalian expression plasmid||HG11275-M-N|
|Human SULT1E1 ORF mammalian expression plasmid, N-Flag tag||HG11275-NF|
|Human SULT1E1 ORF mammalian expression plasmid, N-His tag||HG11275-NH|
|Human SULT1E1 ORF mammalian expression plasmid, N-Myc tag||HG11275-NM|
|Human SULT1E1 ORF mammalian expression plasmid, N-HA tag||HG11275-NY|
|Human SULT1E1 natural ORF mammalian expression plasmid||HG11275-UT|
|Learn more about expression Vectors|
Estrogen sulfotransferase, also known as Sulfotransferase, estrogen-preferring, Sulfotransferase 1E1, SULT1E1 and ST1E1, is a cytoplasm enzyme which belongs to the sulfotransferase 1 family. Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. SULT1E1 may control the level of the estrogen receptor by sulfurylating free estradiol. SULT1E1 maximally sulfates beta-estradiol and estrone at concentrations of 20 nM. SULT1E1 also sulfates dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol and 1-naphthol, at significantly higher concentrations; however, cortisol, testosterone and dopamine are not sulfated. SULT1E1 is a key enzyme in estrogen homeostasis. It plays a central role in the prevention and development of human disease.