|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human FUOM ORF mammalian expression plasmid, C-GFPSpark tag||HG13974-ACG|
|Human FUOM ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13974-ACR|
|Human FUOM ORF mammalian expression plasmid, N-GFPSpark tag||HG13974-ANG|
|Human FUOM ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG13974-ANR|
|Human FUOM ORF mammalian expression plasmid, C-Flag tag||HG13974-CF|
|Human FUOM ORF mammalian expression plasmid, C-His tag||HG13974-CH|
|Human FUOM ORF mammalian expression plasmid, C-Myc tag||HG13974-CM|
|Human FUOM ORF mammalian expression plasmid, C-HA tag||HG13974-CY|
|Human FUOM Gene cDNA clone plasmid||HG13974-G|
|Human FUOM ORF mammalian expression plasmid, N-Flag tag||HG13974-NF|
|Human FUOM ORF mammalian expression plasmid, N-His tag||HG13974-NH|
|Human FUOM ORF mammalian expression plasmid, N-Myc tag||HG13974-NM|
|Human FUOM ORF mammalian expression plasmid, N-HA tag||HG13974-NY|
|Human FUOM natural ORF mammalian expression plasmid||HG13974-UT|
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FUOM, also known as fucose mutarotase and FucM, belongs to the RbsD / FucU family. FUOM is involved in the interconversion between alpha- and beta-L-fucoses. L-Fucose has two isforms: alpha-L-fucose (29.5%) and beta-L-fucose (70.5%). The beta-form is metabolized through the salvage pathway. GDP-L-fucose formed either by the de novo or salvage pathways is transported into the endoplasmic reticulum, where it serves as a substrate for N- and O-glycosylations by fucosyltransferases. Fucosylated structures expressed on cell surfaces or secreted in biological fluids are believed to play a critical role in cell-cell adhesion and recognition processes. FUOM mainly exists as homodimer, but also functions as homotetramer, homooctamer, and homodecamer. FUOM's homodimeric form seems catalytically inactive.