|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human B3GNT6 ORF mammalian expression plasmid, C-GFPSpark tag||HG13951-ACG|
|Human B3GNT6 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13951-ACR|
|Human B3GNT6 ORF mammalian expression plasmid, C-Flag tag||HG13951-CF|
|Human B3GNT6 ORF mammalian expression plasmid, C-His tag||HG13951-CH|
|Human B3GNT6 ORF mammalian expression plasmid, C-Myc tag||HG13951-CM|
|Human B3GNT6 ORF mammalian expression plasmid, C-HA tag||HG13951-CY|
|Human B3GNT6 Gene cDNA clone plasmid||HG13951-G|
|Human B3GNT6 ORF mammalian expression plasmid, N-Flag tag||HG13951-NF|
|Human B3GNT6 ORF mammalian expression plasmid, N-His tag||HG13951-NH|
|Human B3GNT6 ORF mammalian expression plasmid, N-Myc tag||HG13951-NM|
|Human B3GNT6 ORF mammalian expression plasmid, N-HA tag||HG13951-NY|
|Human B3GNT6 natural ORF mammalian expression plasmid||HG13951-UT|
|Learn more about expression Vectors|
B3GNT6 belongs to the glycosyltransferase 31 family. B3GNT6 play s an important role in the synthesis of mucin-type O-glycans in digestive organs. It catalyzes the transfer of GlcNAc from UDP-GlcNAc to GalNAcalpha1-Ser/Thr (Tn antigen) to form the core 3 structure (GlcNAcbeta1-3GalNAcalpha1-Ser/Thr). Core 3 structure exists in O-glycan which is an important precursor in the biosynthesis of mucin-type glycoproteins. Loss of core 3 could lead to the production of secreted mucins, then bacteria would be inefficiently cleared from the system, and chronic inflammation would be developed, which eventually would result in development of cancer. B3GNT6 gene is a tumor suppressor gene.