|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human PPM1G ORF mammalian expression plasmid, C-GFPSpark tag||HG11245-ACG|
|Human PPM1G ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11245-ACR|
|Human PPM1G ORF mammalian expression plasmid, N-GFPSpark tag||HG11245-ANG|
|Human PPM1G ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11245-ANR|
|Human PPM1G ORF mammalian expression plasmid, C-Flag tag||HG11245-CF|
|Human PPM1G ORF mammalian expression plasmid, C-His tag||HG11245-CH|
|Human PPM1G ORF mammalian expression plasmid, C-Myc tag||HG11245-CM|
|Human PPM1G ORF mammalian expression plasmid, C-HA tag||HG11245-CY|
|Human PPM1G Gene cDNA clone plasmid||HG11245-M|
|Human PPM1G ORF mammalian expression plasmid, N-Flag tag||HG11245-NF|
|Human PPM1G ORF mammalian expression plasmid, N-His tag||HG11245-NH|
|Human PPM1G ORF mammalian expression plasmid, N-Myc tag||HG11245-NM|
|Human PPM1G ORF mammalian expression plasmid, N-HA tag||HG11245-NY|
|Human PPM1G natural ORF mammalian expression plasmid||HG11245-UT|
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Protein phosphatase 1G, also known as Protein phosphatase 1C, Protein phosphatase 2C isoform gamma, Protein phosphatase magnesium-dependent 1 gamma, PP2C-gamma, PPM1G and PPM1C, is a cytoplasm protein which belongs to the PP2C family. PPM1G / PP2C-gamma is widely expressed. It is most abundant in testis, skeletal muscle, and heart. Alternatively spliced transcript variants encoding the same protein have been described. PP2C family members are known to be negative regulators of cell stress response pathways. PPM1G / PP2C-gamma is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. PPM1G / PP2C-gamma also plays a role in regulating cell cycle progression.