|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human PSG6 ORF mammalian expression plasmid, C-GFPSpark tag||HG13808-ACG|
|Human PSG6 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13808-ACR|
|Human PSG6 ORF mammalian expression plasmid, C-Flag tag||HG13808-CF|
|Human PSG6 ORF mammalian expression plasmid, C-His tag||HG13808-CH|
|Human PSG6 ORF mammalian expression plasmid, C-Myc tag||HG13808-CM|
|Human PSG6 ORF mammalian expression plasmid, C-HA tag||HG13808-CY|
|Human PSG6 Gene cDNA clone plasmid||HG13808-G|
|Human PSG6 ORF mammalian expression plasmid, N-Flag tag||HG13808-NF|
|Human PSG6 ORF mammalian expression plasmid, N-His tag||HG13808-NH|
|Human PSG6 ORF mammalian expression plasmid, N-Myc tag||HG13808-NM|
|Human PSG6 ORF mammalian expression plasmid, N-HA tag||HG13808-NY|
|Human PSG6 natural ORF mammalian expression plasmid||HG13808-UT|
|Learn more about expression Vectors|
PSG6 is a pregnancy-specific glycoprotein(PSG). PSGs are secreted proteins which are produced by the rodent and primate placenta and play a critical role in pregnancy success. The levels of PSGs are highest during the third trimester of pregnancy, a time marked by the most profound suppression of MS disease attacks. PSGs regulate T-cell function. The regulation of T-cell function during pregnancy is likely the result of significant hormonal changes and may well involve immunoregulatory proteins derived from the placenta. Pregnancy specific glycoproteins (PSGs) are the most abundant placentally derived glycoproteins in the maternal serum. PSG1, PSG6, PSG6N, and PSG11 induce dose-dependent secretion of anti-inflammatory cytokines by human monocytes. Human and murine PSGs exhibit cross-species activity.