|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human RSPO1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11083-ACG|
|Human RSPO1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11083-ACR|
|Human RSPO1 ORF mammalian expression plasmid, C-Flag tag||HG11083-CF|
|Human RSPO1 ORF mammalian expression plasmid, C-His tag||HG11083-CH|
|Human RSPO1 ORF mammalian expression plasmid, C-Myc tag||HG11083-CM|
|Human RSPO1 ORF mammalian expression plasmid, C-HA tag||HG11083-CY|
|Human RSPO1 Gene cDNA clone plasmid||HG11083-M|
|Human RSPO1 ORF mammalian expression plasmid, N-Flag tag||HG11083-NF|
|Human RSPO1 ORF mammalian expression plasmid, N-His tag||HG11083-NH|
|Human RSPO1 ORF mammalian expression plasmid, N-Myc tag||HG11083-NM|
|Human RSPO1 ORF mammalian expression plasmid, N-HA tag||HG11083-NY|
|Human RSPO1 natural ORF mammalian expression plasmid||HG11083-UT|
|Learn more about expression Vectors|
RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6.