|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CNPY2 ORF mammalian expression plasmid, C-GFPSpark tag||HG13771-ACG|
|Human CNPY2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13771-ACR|
|Human CNPY2 ORF mammalian expression plasmid, C-Flag tag||HG13771-CF|
|Human CNPY2 ORF mammalian expression plasmid, C-His tag||HG13771-CH|
|Human CNPY2 ORF mammalian expression plasmid, C-Myc tag||HG13771-CM|
|Human CNPY2 ORF mammalian expression plasmid, C-HA tag||HG13771-CY|
|Human CNPY2 Gene cDNA clone plasmid||HG13771-G|
|Human CNPY2 ORF mammalian expression plasmid, N-Flag tag||HG13771-NF|
|Human CNPY2 ORF mammalian expression plasmid, N-His tag||HG13771-NH|
|Human CNPY2 ORF mammalian expression plasmid, N-Myc tag||HG13771-NM|
|Human CNPY2 ORF mammalian expression plasmid, N-HA tag||HG13771-NY|
|Human CNPY2 natural ORF mammalian expression plasmid||HG13771-UT|
|Learn more about expression Vectors|
CNPY2 is a novel MIR-interacting protein that enhances neurite outgrowth and increases myosin regulatory light chain. CNPY2 enhances migration of C6 glioma cells through phosphorylation of the myosin regulatory light chain. It is expressed in different tissues, including brain. Overexpression of CNPY2 enhanced the motility of glioma cells measured in matrigel invasion chambers and using a scratch assay. Downregulation of CNPY2 by RNA interference significantly decreased glioma cell migration and phosphorylation of MRLC. Inhibition of the corresponding MRLC kinase by ML-7 did not affect migration of CNPY2-overexpressing cells.