|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human SCARB2 ORF mammalian expression plasmid, C-GFPSpark tag||HG11063-ACG|
|Human SCARB2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11063-ACR|
|Human SCARB2 ORF mammalian expression plasmid, N-GFPSpark tag||HG11063-ANG|
|Human SCARB2 ORF mammalian expression plasmid, C-Flag tag||HG11063-CF|
|Human SCARB2 ORF mammalian expression plasmid, C-His tag||HG11063-CH|
|Human SCARB2 ORF mammalian expression plasmid, C-Myc tag||HG11063-CM|
|Human SCARB2 ORF mammalian expression plasmid, C-HA tag||HG11063-CY|
|Human SCARB2 Gene cDNA clone plasmid||HG11063-M|
|Human SCARB2 ORF mammalian expression plasmid, N-Flag tag||HG11063-NF|
|Human SCARB2 ORF mammalian expression plasmid, N-His tag||HG11063-NH|
|Human SCARB2 ORF mammalian expression plasmid, N-Myc tag||HG11063-NM|
|Human SCARB2 ORF mammalian expression plasmid, N-HA tag||HG11063-NY|
|Human SCARB2 natural ORF mammalian expression plasmid||HG11063-UT|
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Lysosomal Integral Membrane Protein II (LIMPII), also known as SCARB2, LPG85, and CD36L2, is a type I II multi-pass membrane glycoprotein that is located primarily in limiting membranes of lysosomes and endosomes on all tissues and cell types so far examined. This protein may participate in membrane transportation and the reorganization of endosomal/lysosomal compartment. LIMPII is identified as a receptor for EV71 (human enterovirus species A, Enterovirus 71) and CVA16 (coxsackievirus A16) which are most frequently associated with hand, foot and mouth disease (HFMD). Expression of human LIMPII enables normally unsusceptible cell lines to support the viruses’ propagation and develop cytopathic effects. In addition, LIMPII also has been shown to bind thrombospondin-1, may contribute to the pro-adhesive changes of activated platelets during coagulation, and inflammation. Deficiency of the protein in mice impairs cell membrane transport processes and causes pelvic junction obstruction, deafness, and peripheral neuropathy.