|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human QPCT ORF mammalian expression plasmid, C-GFPSpark tag||HG13752-ACG|
|Human QPCT ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13752-ACR|
|Human QPCT ORF mammalian expression plasmid, C-Flag tag||HG13752-CF|
|Human QPCT ORF mammalian expression plasmid, C-His tag||HG13752-CH|
|Human QPCT ORF mammalian expression plasmid, C-Myc tag||HG13752-CM|
|Human QPCT ORF mammalian expression plasmid, C-HA tag||HG13752-CY|
|Human QPCT Gene cDNA clone plasmid||HG13752-G|
|Human QPCT ORF mammalian expression plasmid, N-Flag tag||HG13752-NF|
|Human QPCT ORF mammalian expression plasmid, N-His tag||HG13752-NH|
|Human QPCT ORF mammalian expression plasmid, N-Myc tag||HG13752-NM|
|Human QPCT ORF mammalian expression plasmid, N-HA tag||HG13752-NY|
|Human QPCT natural ORF mammalian expression plasmid||HG13752-UT|
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Glutaminyl cyclase, also known as QPCT, can promote the N-terminal cyclization reaction of N-terminal pyroglutamate(pGlu). The pGlu formation from its glutaminyl precursor is required in the maturation of numerous bioactive peptides, while the aberrant formation of pGlu may be related to several pathological processes, such as osteoporosis and amyloidotic diseases. Glutaminyl cyclase's structure reveals an alpha/beta scaffold akin to that of two-zinc exopeptidases but with several insertions and deletions, particularly in the active-site region. Glutaminyl cyclase's amino acid sequence of this enzyme is 86% identical to that of bovine glutaminyl cyclase. It is responsible for the presence of pyroglutamyl residues in many neuroendocrine peptides.