|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human TMEM25 ORF mammalian expression plasmid, C-GFPSpark tag||HG13750-ACG|
|Human TMEM25 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13750-ACR|
|Human TMEM25 ORF mammalian expression plasmid, C-Flag tag||HG13750-CF|
|Human TMEM25 ORF mammalian expression plasmid, C-His tag||HG13750-CH|
|Human TMEM25 ORF mammalian expression plasmid, C-Myc tag||HG13750-CM|
|Human TMEM25 ORF mammalian expression plasmid, C-HA tag||HG13750-CY|
|Human TMEM25 Gene cDNA clone plasmid||HG13750-G|
|Human TMEM25 ORF mammalian expression plasmid, N-Flag tag||HG13750-NF|
|Human TMEM25 ORF mammalian expression plasmid, N-His tag||HG13750-NH|
|Human TMEM25 ORF mammalian expression plasmid, N-Myc tag||HG13750-NM|
|Human TMEM25 ORF mammalian expression plasmid, N-HA tag||HG13750-NY|
|Human TMEM25 natural ORF mammalian expression plasmid||HG13750-UT|
|Learn more about expression Vectors|
TMEM25 is a novel member of the immunoglobulin superfamily. Immunoglobulin superfamily members are implicated in immune responses, growth factor signaling, and cell adhesion. TMEM25 contains 1 Ig-like (immunoglobulin-like) domain and is a target of pharmacogenomics in the field of oncology and regenerative medicine. TMEM25 isoform 1, consisting of exons 1-9, encoded a 366-aa transmembrane protein. TMEM25 isoform 2, consisting of exons 1-4 and 6-9, encoded a 322-aa secreted protein. TMEM25 mRNA was expressed in brain, including cerebellar cortex and hippocampus, as well as in neuroblastoma, brain tumors, and gastric cancer. Human TMEM25 gene was located at the 11q23.3 oncogenomic recombination hotspot around the MLL amplicon and the neuroblastoma deleted region.