|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CSNK2A2 ORF mammalian expression plasmid, C-GFPSpark tag||HG11018-ACG|
|Human CSNK2A2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11018-ACR|
|Human CSNK2A2 ORF mammalian expression plasmid, N-GFPSpark tag||HG11018-ANG|
|Human CSNK2A2 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11018-ANR|
|Human CSNK2A2 ORF mammalian expression plasmid, C-Flag tag||HG11018-CF|
|Human CSNK2A2 ORF mammalian expression plasmid, C-His tag||HG11018-CH|
|Human CSNK2A2 ORF mammalian expression plasmid, C-Myc tag||HG11018-CM|
|Human CSNK2A2 ORF mammalian expression plasmid, C-HA tag||HG11018-CY|
|Human CSNK2A2 Gene cDNA clone plasmid||HG11018-M|
|Human CSNK2A2 ORF mammalian expression plasmid, Flag tag||HG11018-M-F|
|Human CSNK2A2 natural ORF mammalian expression plasmid||HG11018-M-N|
|Human CSNK2A2 ORF mammalian expression plasmid, N-Flag tag||HG11018-NF|
|Human CSNK2A2 ORF mammalian expression plasmid, N-His tag||HG11018-NH|
|Human CSNK2A2 ORF mammalian expression plasmid, N-Myc tag||HG11018-NM|
|Human CSNK2A2 ORF mammalian expression plasmid, N-HA tag||HG11018-NY|
|Human CSNK2A2 natural ORF mammalian expression plasmid||HG11018-UT|
|Learn more about expression Vectors|
Casein kinase II subunit alpha', also known as CSNK2A2 and CK2A2, is a member of the protein kinase superfamily, Ser/Thr protein kinase family and CK2 subfamily. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. CSNK2A2 is a tetramer composed of an alpha chain, an alpha' and two beta chains. It is also component of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, the complex associating following UV irradiation. Protein kinase casein kinase II (Ck2) is a cyclic-AMP and calcium-independent serine-threonine kinase that is composed of two catalytic subunits (alpha and alpha') and two regulatory beta-subunits. Ck2 is not a casein kinase in vivo, but over 100 substrates are known. The highly conserved amino acid sequences of its subunits and their broad expression suggest that Ck2 may have a fundamental role in cell function. Ck2 has been implicated in DNA replication, regulation of basal and inducible transcription, translation and control of metabolism. The Ck2alpha and Ck2alpha' isoforms (products of the genes Csnk2a1 and Csnk2a2, respectively) are highly homologous, the reason for their redundancy and evolutionary conservation is unknown. CSNK2A2 may be a candidate gene for these inherited syndromes.