|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human SERPINA6 ORF mammalian expression plasmid, C-GFPSpark tag||HG10998-ACG|
|Human SERPINA6 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10998-ACR|
|Human SERPINA6 ORF mammalian expression plasmid, C-Flag tag||HG10998-CF|
|Human SERPINA6 ORF mammalian expression plasmid, C-His tag||HG10998-CH|
|Human SERPINA6 ORF mammalian expression plasmid, C-Myc tag||HG10998-CM|
|Human SERPINA6 ORF mammalian expression plasmid, C-HA tag||HG10998-CY|
|Human SERPINA6 Gene cDNA clone plasmid||HG10998-M|
|Human SERPINA6 ORF mammalian expression plasmid, Flag tag||HG10998-M-F|
|Human SERPINA6 ORF mammalian expression plasmid, N-Flag tag||HG10998-NF|
|Human SERPINA6 ORF mammalian expression plasmid, N-His tag||HG10998-NH|
|Human SERPINA6 ORF mammalian expression plasmid, N-Myc tag||HG10998-NM|
|Human SERPINA6 ORF mammalian expression plasmid, N-HA tag||HG10998-NY|
|Human SERPINA6 natural ORF mammalian expression plasmid||HG10998-UT|
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Corticosteroid-binding globulin (CBG), also known as SerpinA6, is a non-inhibitory member of the serine proteinase inhibitor (serpin) superfamily. It is the high-affinity transport protein for glucocorticoids in vertebrate blood. CBG is specifically cleaved by this protease at a precise site close to its carboxy-terminus. This induces a conformation change and disrupts the binding between glucocorticoids and CBG, and promotes a significant and local release of glucocorticoids (over 90% of them are bound to CBG in human plasma). In this context, CBG directs glucocorticoids to sites of inflammation, and plays in consequence a crucial role in efficient glucocorticoid action in physiology. The SerpinA6 protein is mainly secreted by the liver. This negative acute phase protein regulates free cortisol levels in the blood and distributes cortisol to its target tissues. SerpinA6 deficiency is an extremely rare hereditary disorder characterized by reduced corticosteroid-binding capacity with normal or low plasma corticosteroid-binding globulin concentration, and normal or low basal cortisol levels associated with hypo-/hypertension and muscle fatigue. There are three heritable, human CBG gene mutations that can reduce CBG-cortisol binding affinity and/or reduce circulating CBG levels.