|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human SERPINA7 ORF mammalian expression plasmid, C-GFPSpark tag||HG10993-ACG|
|Human SERPINA7 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10993-ACR|
|Human SERPINA7 ORF mammalian expression plasmid, C-Flag tag||HG10993-CF|
|Human SERPINA7 ORF mammalian expression plasmid, C-His tag||HG10993-CH|
|Human SERPINA7 ORF mammalian expression plasmid, C-Myc tag||HG10993-CM|
|Human SERPINA7 ORF mammalian expression plasmid, C-HA tag||HG10993-CY|
|Human SERPINA7 Gene cDNA clone plasmid||HG10993-M|
|Human SERPINA7 ORF mammalian expression plasmid, N-Flag tag||HG10993-NF|
|Human SERPINA7 ORF mammalian expression plasmid, N-His tag||HG10993-NH|
|Human SERPINA7 ORF mammalian expression plasmid, N-Myc tag||HG10993-NM|
|Human SERPINA7 ORF mammalian expression plasmid, N-HA tag||HG10993-NY|
|Human SERPINA7 natural ORF mammalian expression plasmid||HG10993-UT|
|Learn more about expression Vectors|
Thyroxine-binding globulin, also known as T4-binding globulin, Serpin A7 and TBG, is a secreted protein which belongs to the serpin family. TBG is synthesized primarily in the liver as a 54 kDa protein.TBG is genomically a serpin, although it has no inhibitory function like many other members of this class of proteins. TBG binds thyroid hormone in circulation. It is one of three proteins (along with transthyretin and albumin) responsible for carrying the thyroid hormones thyroxine (T4) and 3,5,3’-triiodothyronine (T3) in the bloodstream. Of these three proteins, TBG has the highest affinity for T4 and T3, but is present in the lowest concentration. Despite its low concentration, TBG carries the majority of T4 in serum. Due to the very low serum concentration of T4 and T3, TBG is rarely more than 25% saturated with its ligand. Unlike transthyretin and albumin, TBG has a single binding site for T4/T3. TBG tests are sometimes used in finding the reason for elevated or diminished levels of thyroid hormone.