|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CD8A ORF mammalian expression plasmid, C-GFPSpark tag||HG10980-ACG|
|Human CD8A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10980-ACR|
|Human CD8A ORF mammalian expression plasmid, C-Flag tag||HG10980-CF|
|Human CD8A ORF mammalian expression plasmid, C-His tag||HG10980-CH|
|Human CD8A ORF mammalian expression plasmid, C-Myc tag||HG10980-CM|
|Human CD8A ORF mammalian expression plasmid, C-HA tag||HG10980-CY|
|Human CD8A Gene cDNA clone plasmid||HG10980-M|
|Human CD8A ORF mammalian expression plasmid, N-Flag tag||HG10980-NF|
|Human CD8A ORF mammalian expression plasmid, N-His tag||HG10980-NH|
|Human CD8A ORF mammalian expression plasmid, N-Myc tag||HG10980-NM|
|Human CD8A ORF mammalian expression plasmid, N-HA tag||HG10980-NY|
|Human CD8A natural ORF mammalian expression plasmid||HG10980-UT|
|Learn more about expression Vectors|
Human T-cell surface glycoprotein CD8 alpha chain, also known as CD8a, is a single-pass type I membrane protein. The CD8 glycoprotein is expressed by thymocytes, mature T cells and natural killer (NK) cells and has been implicated in the recognition of monomorphic determinants on major histocompatibility complex (MHC) Class I antigens, and in signal transduction during the course of T-cell activation. Both human and rodent CD8 antigens are comprised of two distinct polypeptide chains, alpha and beta. The Ig domains of CD8 alpha are involved in controlling the ability of CD8 to be expressed. Mutation of B- and F-strand cysteine residues in CD8 alpha reduced the ability of the protein to fold properly and, therefore, to be expressed. Defects in CD8A are a cause of familial CD8 deficiency. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.