|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human IL6ST ORF mammalian expression plasmid, C-GFPSpark tag||HG10974-ACG|
|Human IL6ST ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10974-ACR|
|Human IL6ST ORF mammalian expression plasmid, C-Flag tag||HG10974-CF|
|Human IL6ST ORF mammalian expression plasmid, C-His tag||HG10974-CH|
|Human IL6ST ORF mammalian expression plasmid, C-Myc tag||HG10974-CM|
|Human IL6ST ORF mammalian expression plasmid, C-HA tag||HG10974-CY|
|Human IL6ST Gene cDNA clone plasmid||HG10974-M|
|Human IL6ST ORF mammalian expression plasmid, N-Flag tag||HG10974-NF|
|Human IL6ST ORF mammalian expression plasmid, N-His tag||HG10974-NH|
|Human IL6ST ORF mammalian expression plasmid, N-Myc tag||HG10974-NM|
|Human IL6ST ORF mammalian expression plasmid, N-HA tag||HG10974-NY|
|Human IL6ST natural ORF mammalian expression plasmid||HG10974-UT|
|Learn more about expression Vectors|
Glycoprotein 130 (also known as gp130, IL6ST, IL6-beta or CD130) is a transmembrane protein which is the founding member of the class of all cytokine receptors. CD130/gp130 is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and Oncostatin M (OSM). CD130/gp130 functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. CD130/gp130 plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. A related pseudogene has been identified on chromosome 17. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. CD130/gp130 binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. CD130/gp130 may have a role in embryonic development. The type I OSM receptor is capable of transducing OSM-specific signaling events.