|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human STATH ORF mammalian expression plasmid, C-GFPSpark tag||HG13694-ACG|
|Human STATH ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13694-ACR|
|Human STATH ORF mammalian expression plasmid, C-Flag tag||HG13694-CF|
|Human STATH ORF mammalian expression plasmid, C-His tag||HG13694-CH|
|Human STATH ORF mammalian expression plasmid, C-Myc tag||HG13694-CM|
|Human STATH ORF mammalian expression plasmid, C-HA tag||HG13694-CY|
|Human STATH Gene cDNA clone plasmid||HG13694-G|
|Human STATH ORF mammalian expression plasmid, N-Flag tag||HG13694-NF|
|Human STATH ORF mammalian expression plasmid, N-His tag||HG13694-NH|
|Human STATH ORF mammalian expression plasmid, N-Myc tag||HG13694-NM|
|Human STATH ORF mammalian expression plasmid, N-HA tag||HG13694-NY|
|Human STATH natural ORF mammalian expression plasmid||HG13694-UT|
|Learn more about expression Vectors|
Statherin, also known as STATH, belongs to the histatin/statherin family. Statherin may play an important role in the maintenance of oral health.It prevents calcium phospate precipitation in saliva, so maintaining a high calcium level in saliva and preventing teeth from dissolving. Statherin also inhibits spontaneous precipitation of calcium phosphate salts. Thus, statherin and PRPs may prevent build-up of harmful deposits in the salivary glands and on the tooth surfaces.Statherin is a highly stable salivary protein of low molecular mass (5,380). Sabatini et al. synthesized mixed oligonucleotides based on the known amino acid sequence of statherin and used these to screen a cDNA library constructed from human parotid gland mRNA.