|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human PNLIPRP2 ORF mammalian expression plasmid, C-GFPSpark tag||HG13687-ACG|
|Human PNLIPRP2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13687-ACR|
|Human PNLIPRP2 ORF mammalian expression plasmid, C-Flag tag||HG13687-CF|
|Human PNLIPRP2 ORF mammalian expression plasmid, C-His tag||HG13687-CH|
|Human PNLIPRP2 ORF mammalian expression plasmid, C-Myc tag||HG13687-CM|
|Human PNLIPRP2 ORF mammalian expression plasmid, C-HA tag||HG13687-CY|
|Human PNLIPRP2 Gene cDNA clone plasmid||HG13687-G|
|Human PNLIPRP2 ORF mammalian expression plasmid, N-Flag tag||HG13687-NF|
|Human PNLIPRP2 ORF mammalian expression plasmid, N-His tag||HG13687-NH|
|Human PNLIPRP2 ORF mammalian expression plasmid, N-Myc tag||HG13687-NM|
|Human PNLIPRP2 ORF mammalian expression plasmid, N-HA tag||HG13687-NY|
|Human PNLIPRP2 natural ORF mammalian expression plasmid||HG13687-UT|
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Galactolipase, also known as PNLIPRP2, is a lipase with broad substrate specificity. It can hydrolyze both phospholipids and galactolipids. Galactolipase acts preferentially on monoglycerides, phospholipids and galactolipids. It also hydrolyses milk fat with a lower catalytic efficiency. The expressed galactolipase shows a lipolytic activity that is, however, only marginally dependent on the presence of colipase. The lipolytic activity of pancreatic extracts and human pancreatic juice on Labrasol is mainly due to the combined action of carboxyl ester hydrolase and galactolipase.