|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human IL34 ORF mammalian expression plasmid, C-GFPSpark tag||HG10948-ACG|
|Human IL34 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10948-ACR|
|Human IL34 ORF mammalian expression plasmid, C-Flag tag||HG10948-CF|
|Human IL34 ORF mammalian expression plasmid, C-His tag||HG10948-CH|
|Human IL34 ORF mammalian expression plasmid, C-Myc tag||HG10948-CM|
|Human IL34 ORF mammalian expression plasmid, C-HA tag||HG10948-CY|
|Human IL34 Gene cDNA clone plasmid||HG10948-M|
|Human IL34 ORF mammalian expression plasmid, N-Flag tag||HG10948-NF|
|Human IL34 ORF mammalian expression plasmid, N-His tag||HG10948-NH|
|Human IL34 ORF mammalian expression plasmid, N-Myc tag||HG10948-NM|
|Human IL34 ORF mammalian expression plasmid, N-HA tag||HG10948-NY|
|Human IL34 natural ORF mammalian expression plasmid||HG10948-UT|
|Learn more about expression Vectors|
Interleukin 34 (IL-34), also known as uncharacterized protein C16 or f77 homolog, is a secreted cytokine that belongs to unknown cytokine family. IL-34 protein is expressed in various tissues, including heart, brain, lung, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate and colon, and most abundant in the spleen. The colony-stimulating factor 1 receptor (CSF-1R) is identified as the receptor for IL-34. IL-34 increases growth or survival of immune cells known as monocytes. In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures.