|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human GNGT1 ORF mammalian expression plasmid, C-GFPSpark tag||HG13658-ACG|
|Human GNGT1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13658-ACR|
|Human GNGT1 ORF mammalian expression plasmid, N-GFPSpark tag||HG13658-ANG|
|Human GNGT1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG13658-ANR|
|Human GNGT1 ORF mammalian expression plasmid, C-Flag tag||HG13658-CF|
|Human GNGT1 ORF mammalian expression plasmid, C-His tag||HG13658-CH|
|Human GNGT1 ORF mammalian expression plasmid, C-Myc tag||HG13658-CM|
|Human GNGT1 ORF mammalian expression plasmid, C-HA tag||HG13658-CY|
|Human GNGT1 Gene cDNA clone plasmid||HG13658-G|
|Human GNGT1 ORF mammalian expression plasmid, N-Flag tag||HG13658-NF|
|Human GNGT1 ORF mammalian expression plasmid, N-His tag||HG13658-NH|
|Human GNGT1 ORF mammalian expression plasmid, N-Myc tag||HG13658-NM|
|Human GNGT1 ORF mammalian expression plasmid, N-HA tag||HG13658-NY|
|Human GNGT1 natural ORF mammalian expression plasmid||HG13658-UT|
|Learn more about expression Vectors|
GNGT1 is a subunit of of transducin. Heterotrimeric G proteins consist of alpha, beta, and gamma subunits. They are membrane bound GTPases that are linked to 7-TM receptors. They function as signal transducers for the 7-transmembrane-helix G protein-coupled receptors. They are involved as a modulator or transducer in various transmembrane signaling systems. G proteins are bound to GDP in the 'off' state. GNGT1 is the gamma subunit of transducin. Ligand-receptor binding results in detachment of the G protein, switching it to an 'on' state and permitting Galpha activation of second messenger signalling cascades. There are several types of Galpha proteins; in addition, some Gbetagamma subunits have active functions. Gbetagamma coupled to H1 receptors can activate PLA2 and Gbetagamma coupled to M1 receptors can activate KIR channels. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.