|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human NT5E ORF mammalian expression plasmid, C-GFPSpark tag||HG10904-ACG|
|Human NT5E ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10904-ACR|
|Human NT5E ORF mammalian expression plasmid, C-Flag tag||HG10904-CF|
|Human NT5E ORF mammalian expression plasmid, C-His tag||HG10904-CH|
|Human NT5E ORF mammalian expression plasmid, C-Myc tag||HG10904-CM|
|Human NT5E ORF mammalian expression plasmid, C-HA tag||HG10904-CY|
|Human NT5E Gene cDNA clone plasmid||HG10904-M|
|Human NT5E natural ORF mammalian expression plasmid, HA tag||HG10904-M-Y|
|Human NT5E ORF mammalian expression plasmid, N-Flag tag||HG10904-NF|
|Human NT5E ORF mammalian expression plasmid, N-His tag||HG10904-NH|
|Human NT5E ORF mammalian expression plasmid, N-Myc tag||HG10904-NM|
|Human NT5E ORF mammalian expression plasmid, N-HA tag||HG10904-NY|
|Human NT5E natural ORF mammalian expression plasmid||HG10904-UT|
|Learn more about expression Vectors|
5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein which belongs to the 5'-nucleotidase family. CD73 is a glycosyl phosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signalling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1 and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purigenic receptors to induce cellular responses. CD73 has also been reported to regulate expression of pro-inflammatory molecules in mouse endothelium.