|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human BAMBI / NMA ORF mammalian expression plasmid, C-GFPSpark tag||HG10890-ACG|
|Human BAMBI / NMA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10890-ACR|
|Human BAMBI / NMA ORF mammalian expression plasmid, C-Flag tag||HG10890-CF|
|Human BAMBI / NMA ORF mammalian expression plasmid, C-His tag||HG10890-CH|
|Human BAMBI / NMA ORF mammalian expression plasmid, C-Myc tag||HG10890-CM|
|Human BAMBI / NMA ORF mammalian expression plasmid, C-HA tag||HG10890-CY|
|Human BAMBI / NMA Gene cDNA clone plasmid||HG10890-M|
|Human BAMBI / NMA ORF mammalian expression plasmid, N-Flag tag||HG10890-NF|
|Human BAMBI / NMA ORF mammalian expression plasmid, N-His tag||HG10890-NH|
|Human BAMBI / NMA ORF mammalian expression plasmid, N-Myc tag||HG10890-NM|
|Human BAMBI / NMA ORF mammalian expression plasmid, N-HA tag||HG10890-NY|
|Human BAMBI / NMA natural ORF mammalian expression plasmid||HG10890-UT|
|Learn more about expression Vectors|
BMP and activin membrane-bound inhibitor (BAMBI) is a transmembrane glycoprotein that is a pseudoreceptor of type 1 receptors. BAMBI structurally lacks intracellular serine/ threonine kinase domain but with an extracellular domain and a short cytoplasmic region that share sequence similarities with type 1 receptors, whose members have functions in signal transduction in various developing and pathological processes. BAMBI competes with the type 1 receptor, a receptor of the transforming growth factor-beta (TGF-beta), through functioning as negative regulators of TGF-beta by limiting the signaling range of the TGF-beta family during early embryogenesis. The expression of BAMBI can be induced by accumulated beta-catenin and BMP. The expression level of BAMBI was found aberrantly elevated in most colorectal and hepatocellular carcinomas relative to the corresponding non-cancerous tissues. It suggestes that beta-catenin and TGF-beta interfere growth arrest by inducing the expression of BAMBI, and this may contribute to colorectal and hepatocellular tumorigenesis.