|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CXCL5 ORF mammalian expression plasmid, C-GFPSpark tag||HG10889-ACG|
|Human CXCL5 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10889-ACR|
|Human CXCL5 ORF mammalian expression plasmid, C-Flag tag||HG10889-CF|
|Human CXCL5 ORF mammalian expression plasmid, C-His tag||HG10889-CH|
|Human CXCL5 ORF mammalian expression plasmid, C-Myc tag||HG10889-CM|
|Human CXCL5 ORF mammalian expression plasmid, C-HA tag||HG10889-CY|
|Human CXCL5 Gene cDNA clone plasmid||HG10889-M|
|Human CXCL5 ORF mammalian expression plasmid, Flag tag||HG10889-M-F|
|Human CXCL5 ORF mammalian expression plasmid, N-Flag tag||HG10889-NF|
|Human CXCL5 ORF mammalian expression plasmid, N-His tag||HG10889-NH|
|Human CXCL5 ORF mammalian expression plasmid, N-Myc tag||HG10889-NM|
|Human CXCL5 ORF mammalian expression plasmid, N-HA tag||HG10889-NY|
|Human CXCL5 natural ORF mammalian expression plasmid||HG10889-UT|
|Learn more about expression Vectors|
CXCL5 is a small cytokine belonging to the CXC chemokine family. CXC chemokines are particularly significant for leukocyte infiltration in inflammatory diseases. CXCL5 is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. It also can be detected in eosinophils, and can be inhibited with the type II interferon. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis. It stimulates the chemotaxis of neutrophils possesses angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2.