|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CXCL9 ORF mammalian expression plasmid, C-GFPSpark tag||HG10888-ACG|
|Human CXCL9 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10888-ACR|
|Human CXCL9 ORF mammalian expression plasmid, C-Flag tag||HG10888-CF|
|Human CXCL9 ORF mammalian expression plasmid, C-His tag||HG10888-CH|
|Human CXCL9 ORF mammalian expression plasmid, C-Myc tag||HG10888-CM|
|Human CXCL9 ORF mammalian expression plasmid, C-HA tag||HG10888-CY|
|Human CXCL9 Gene cDNA clone plasmid||HG10888-M|
|Human CXCL9 ORF mammalian expression plasmid, Flag tag||HG10888-M-F|
|Human CXCL9 ORF mammalian expression plasmid, His tag||HG10888-M-H|
|Human CXCL9 ORF mammalian expression plasmid, N-Flag tag||HG10888-NF|
|Human CXCL9 ORF mammalian expression plasmid, N-His tag||HG10888-NH|
|Human CXCL9 ORF mammalian expression plasmid, N-Myc tag||HG10888-NM|
|Human CXCL9 ORF mammalian expression plasmid, N-HA tag||HG10888-NY|
|Human CXCL9 natural ORF mammalian expression plasmid||HG10888-UT|
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Chemokine (C-X-C motif) ligand 9 (CXCL9), also known as Monokine induced by gamma interferon (MIG), is a small cytokine belonging to the CXC chemokine family. The function of this chemokine has not been specifically defined; however, it is thought to be involved in T cell trafficking. CXCL9/MIG functions as one of the three ligands of chemokine receptor CXCR3 which is a G protein-coupled receptor found predominantly on T cells. CXCL9/MIG, together with CXCL10 and CXCL11, may activate CXCR3 by binding to it. CXCL9 serves as a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. It has been observed that tumour endothelial cells secrete high levels of CXCL9 in all, and CXCL10 in most melanoma metastases. Experiment data represent novel mechanisms by which tumour cells in melanoma metastases might use the chemokine-expressing endothelium to leave the tumour and eventually to form additional metastases at distinct sites. Experiment results also improved that CXCL9/MIG plays an important role in CD4+ T lymphocyte recruitment and development of CAV, MOMA-2+ macrophages are the predominant recipient-derived source of CXCL9/MIG, and recipient CD4 lymphocytes are necessary for sustained CXCL9/MIG production and CAV development in this model. Neutralization of the chemokine CXCL9/MIG may have therapeutic potential for the treatment of chronic rejection after heart transplantation.