|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CD200 ORF mammalian expression plasmid, C-GFPSpark tag||HG10886-ACG|
|Human CD200 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10886-ACR|
|Human CD200 ORF mammalian expression plasmid, C-Flag tag||HG10886-CF|
|Human CD200 ORF mammalian expression plasmid, C-His tag||HG10886-CH|
|Human CD200 ORF mammalian expression plasmid, C-Myc tag||HG10886-CM|
|Human CD200 ORF mammalian expression plasmid, C-HA tag||HG10886-CY|
|Human CD200 Gene cDNA clone plasmid||HG10886-M|
|Human CD200 ORF mammalian expression plasmid, N-Flag tag||HG10886-NF|
|Human CD200 ORF mammalian expression plasmid, N-His tag||HG10886-NH|
|Human CD200 ORF mammalian expression plasmid, N-Myc tag||HG10886-NM|
|Human CD200 ORF mammalian expression plasmid, N-HA tag||HG10886-NY|
|Human CD200 natural ORF mammalian expression plasmid||HG10886-UT|
|Learn more about expression Vectors|
CD200 (OX-2) is a cell surface glycoprotein that imparts immune privileges by suppressing alloimmune and autoimmune responses through its receptor, CD200R, expressed primarily on myeloid cells. Signals delivered through the CD200:CD200R axis have been shown to play an important role in the regulation of anti-tumor immunity, and overexpression of CD200 has been reported in a number of malignancies, including CLL, as well as on cancer stem cells. The role of CD200-CD200R signaling in immune regulation of the central nervous system has become a popular field of research in recent years. Many studies have shown that there is a close correlation between CD200-CD200R, microglia activation, and Parkinson's disease (PD). The ability of CD200 to suppress myeloid cell activation is critical for maintaining normal tissue homeostasis but may also enhance the survival of migratory neoplastic cells. CD200 and CD200R associate via their respective N-terminal Ig-like domains. CD200 has been characterized as an important immunoregulatory molecule, increased expression of which can lead to decreased transplant rejection, autoimmunity, and allergic disease. Elevated CD200 expression has been reported to be associated with poor prognosis in a number of human malignancies. In addition, CD200 also plays an important role in prevention of graft rejection, autoimmune diseases and spontaneous abortion.