|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CXCL11 ORF mammalian expression plasmid, C-GFPSpark tag||HG10876-ACG|
|Human CXCL11 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10876-ACR|
|Human CXCL11 ORF mammalian expression plasmid, C-Flag tag||HG10876-CF|
|Human CXCL11 ORF mammalian expression plasmid, C-His tag||HG10876-CH|
|Human CXCL11 ORF mammalian expression plasmid, C-Myc tag||HG10876-CM|
|Human CXCL11 ORF mammalian expression plasmid, C-HA tag||HG10876-CY|
|Human CXCL11 Gene cDNA clone plasmid||HG10876-M|
|Human CXCL11 ORF mammalian expression plasmid, Flag tag||HG10876-M-F|
|Human CXCL11 ORF mammalian expression plasmid, N-Flag tag||HG10876-NF|
|Human CXCL11 ORF mammalian expression plasmid, N-His tag||HG10876-NH|
|Human CXCL11 ORF mammalian expression plasmid, N-Myc tag||HG10876-NM|
|Human CXCL11 ORF mammalian expression plasmid, N-HA tag||HG10876-NY|
|Human CXCL11 natural ORF mammalian expression plasmid||HG10876-UT|
|Learn more about expression Vectors|
I-TAC, also known as CXCL11, is a small cytokine belonging to the CXC chemokine family. It is highly expressed in peripheral blood leukocytes, pancreas and liver, with moderate levels in thymus, spleen and lung and low expression levels were in small intestine, placenta and prostate. The I-TAC chemokine elicits its effects on its target cells by interacting with the cell surface chemokine receptor CXCR3, with a higher affinity than do the other ligands for this receptor, CXCL9 and CXCL10. I-TAC is chemotactic for activated T cells. The CXCL11 gene is located on human chromosome 4 along with many other members of the CXC chemokine family.