|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human PDE1C ORF mammalian expression plasmid, C-GFPSpark tag||HG13605-ACG|
|Human PDE1C ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13605-ACR|
|Human PDE1C ORF mammalian expression plasmid, N-GFPSpark tag||HG13605-ANG|
|Human PDE1C ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG13605-ANR|
|Human PDE1C ORF mammalian expression plasmid, C-Flag tag||HG13605-CF|
|Human PDE1C ORF mammalian expression plasmid, C-His tag||HG13605-CH|
|Human PDE1C ORF mammalian expression plasmid, C-Myc tag||HG13605-CM|
|Human PDE1C ORF mammalian expression plasmid, C-HA tag||HG13605-CY|
|Human PDE1C Gene cDNA clone plasmid||HG13605-G|
|Human PDE1C ORF mammalian expression plasmid, N-Flag tag||HG13605-NF|
|Human PDE1C ORF mammalian expression plasmid, N-His tag||HG13605-NH|
|Human PDE1C ORF mammalian expression plasmid, N-Myc tag||HG13605-NM|
|Human PDE1C ORF mammalian expression plasmid, N-HA tag||HG13605-NY|
|Human PDE1C natural ORF mammalian expression plasmid||HG13605-UT|
|Learn more about expression Vectors|
PDE1C belongs to the cyclic nucleotide phosphodiesterase family, PDE1 subfamily. Phosphodiesterases (PDEs) are a family of related phosphohydrolyases that selectively catalyze the hydrolysis of 3' cyclic phosphate bonds in adenosine and/or guanine 3',5' cyclic monophosphate (cAMP and/or cGMP). They regulate the cellular levels, localization and duration of action of these second messengers by controlling the rate of their degradation. PDEs are expressed ubiquitously, with each subtype having a specific tissue distribution. These enzymes are involved in many signal transduction pathways and their functions include vascular smooth muscle proliferation and contraction, cardiac contractility, platelet aggregation, hormone secretion, immune cell activation, and they are involved in learning and memory. PDE1C has a high affinity for both cAMP and cGMP. It is expressed in several tissues, including brain and heart. As a cyclic nucleotide phosphodiesterase, PDE1C has a dual-specificity for the second messengers cAMP and cGMP.