|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human GPR114 ORF mammalian expression plasmid, C-GFPSpark tag||HG10854-ACG|
|Human GPR114 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10854-ACR|
|Human GPR114 ORF mammalian expression plasmid, C-Flag tag||HG10854-CF|
|Human GPR114 ORF mammalian expression plasmid, C-His tag||HG10854-CH|
|Human GPR114 ORF mammalian expression plasmid, C-Myc tag||HG10854-CM|
|Human GPR114 ORF mammalian expression plasmid, C-HA tag||HG10854-CY|
|Human GPR114 Gene cDNA clone plasmid||HG10854-M|
|Human GPR114 natural ORF mammalian expression plasmid||HG10854-M-N|
|Human GPR114 ORF mammalian expression plasmid, N-Flag tag||HG10854-NF|
|Human GPR114 ORF mammalian expression plasmid, N-His tag||HG10854-NH|
|Human GPR114 ORF mammalian expression plasmid, N-Myc tag||HG10854-NM|
|Human GPR114 ORF mammalian expression plasmid, N-HA tag||HG10854-NY|
|Human GPR114 natural ORF mammalian expression plasmid||HG10854-UT|
|Learn more about expression Vectors|
GPR114 belongs to the G-protein coupled receptor 2 family. Members of this family share a common molecular architecture which consists of seven transmembrane domains, three extracellular loops, three intracellular loops, an amino-terminal extracellular domain and an intracellular carboxyl terminus. It is thought that light acts as the activating stimulus of a G-protein-coupled receptor (GPCR). GPCRs are expected to have molecular function (G-protein coupled receptor activity) and to localize in various compartments (endoplasmic reticulum membrane, plasma membrane, integral to membrane). Family B of the GPCRs is a small but structurally and functionally diverse group of proteins that includes receptors for polypeptide hormones, molecules thought to mediate intercellular interactions at the plasma membrane and a group of Drosophila proteins that regulate stress responses and longevity. GPR114 contains 1 GPS domain. GPR114 gene has been proposed to participate in processes (G-protein coupled receptor protein signaling pathway, neuropeptide signaling pathway).