|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human DEFB103A ORF mammalian expression plasmid, C-GFPSpark tag||HG10849-ACG|
|Human DEFB103A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10849-ACR|
|Human DEFB103A ORF mammalian expression plasmid, C-Flag tag||HG10849-CF|
|Human DEFB103A ORF mammalian expression plasmid, C-His tag||HG10849-CH|
|Human DEFB103A ORF mammalian expression plasmid, C-Myc tag||HG10849-CM|
|Human DEFB103A ORF mammalian expression plasmid, C-HA tag||HG10849-CY|
|Human DEFB103A Gene cDNA clone plasmid||HG10849-M|
|Human DEFB103A ORF mammalian expression plasmid, Flag tag||HG10849-M-F|
|Human DEFB103A ORF mammalian expression plasmid, N-Flag tag||HG10849-NF|
|Human DEFB103A ORF mammalian expression plasmid, N-His tag||HG10849-NH|
|Human DEFB103A ORF mammalian expression plasmid, N-Myc tag||HG10849-NM|
|Human DEFB103A ORF mammalian expression plasmid, N-HA tag||HG10849-NY|
|Human DEFB103A natural ORF mammalian expression plasmid||HG10849-UT|
|Learn more about expression Vectors|
Beta-defensin 3 is a member of the defensin family. Defensin family is comprised by microbicidal and cytotoxic peptides made by neutrophils. Members of the beta-defensin 3 family are highly similar in protein sequence. Beta-defensin 3 shows antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Beta-defensin 3 is abundantly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. It is also expressed in salivary gland, bone marrow, colon, stomach, polyp and larynx. However, in small intestine, it cannot be detected. Defensin has broad spectrum antimicrobial activity and may play an important role in innate epithelial defense. Beta-defensin 3 kills multiresistant S.aureus and vancomycin-resistent E.faecium. It has no significant hemolytic activity.