|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human PLA2G7 ORF mammalian expression plasmid, C-GFPSpark tag||HG10848-ACG|
|Human PLA2G7 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10848-ACR|
|Human PLA2G7 ORF mammalian expression plasmid, C-Flag tag||HG10848-CF|
|Human PLA2G7 ORF mammalian expression plasmid, C-His tag||HG10848-CH|
|Human PLA2G7 ORF mammalian expression plasmid, C-Myc tag||HG10848-CM|
|Human PLA2G7 ORF mammalian expression plasmid, C-HA tag||HG10848-CY|
|Human PLA2G7 Gene cDNA clone plasmid||HG10848-M|
|Human PLA2G7 ORF mammalian expression plasmid, N-Flag tag||HG10848-NF|
|Human PLA2G7 ORF mammalian expression plasmid, N-His tag||HG10848-NH|
|Human PLA2G7 ORF mammalian expression plasmid, N-Myc tag||HG10848-NM|
|Human PLA2G7 ORF mammalian expression plasmid, N-HA tag||HG10848-NY|
|Human PLA2G7 natural ORF mammalian expression plasmid||HG10848-UT|
|Learn more about expression Vectors|
Platelet-activating factor acetylhydrolase, also known as 1-alkyl-2-acetylglycerophosphocholine esterase, 2-acetyl-1-alkylglycero-phosphocholine esterase, Group-VIIA phospholipase A2, LDL-associated phospholipase A2, PAF 2-acylhydrolase, PLA2G7 and PAFAH, is secreted protein which belongs to the AB hydrolase superfamily and Lipase family. PLA2G7 / PAFAH modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. It has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids. PLA2G7 / PAFAH is a potent pro- and anti-inflammatory molecule that has been implicated in multiple inflammatory disease processes, including cardiovascular disease. PLA2G7 also represents an important, potentially functional candidate in the pathophysiology of coronary artery disease (CAD). Defects in PLA2G7 are the cause of platelet-activating factor acetylhydrolase deficiency (PLA2G7 deficiency). It is a trait which is present in 27% of Japanese. It could have a significant physiologic effect in the presence of inflammatory bodily responses.