|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human Soggy-1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10840-ACG|
|Human Soggy-1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10840-ACR|
|Human Soggy-1 ORF mammalian expression plasmid, C-Flag tag||HG10840-CF|
|Human Soggy-1 ORF mammalian expression plasmid, C-His tag||HG10840-CH|
|Human Soggy-1 ORF mammalian expression plasmid, C-Myc tag||HG10840-CM|
|Human Soggy-1 ORF mammalian expression plasmid, C-HA tag||HG10840-CY|
|Human Soggy-1 Gene cDNA clone plasmid||HG10840-M|
|Human Soggy-1 natural ORF mammalian expression plasmid||HG10840-M-N|
|Human Soggy-1 ORF mammalian expression plasmid, N-Flag tag||HG10840-NF|
|Human Soggy-1 ORF mammalian expression plasmid, N-His tag||HG10840-NH|
|Human Soggy-1 ORF mammalian expression plasmid, N-Myc tag||HG10840-NM|
|Human Soggy-1 ORF mammalian expression plasmid, N-HA tag||HG10840-NY|
|Human Soggy-1 natural ORF mammalian expression plasmid||HG10840-UT|
|Learn more about expression Vectors|
Dickkopf-like 1 (DKKL1) or soggy 1, is a glycoprotein unique to mammals that is expressed primarily in developing spermatocytes and localized in the acrosome of mature sperm. It is also expressed in the trophectoderm / placental lineage. This glycoprotein is secreted by postmeiotic male germ cells. DKKL1 is a member of the Dickkopf (DKK) family, a group of proteins that are characterized as secreted antagonists of Wnt signal transduction proteins. In mammals, embryos lacking DKKL1 protein developed into viable, fertile adults. DKKL1, either directly or indirectly, facilitates the ability of sperm to penetrate the zona pellucid. DKKL1 is related to the sperm apoptotic procession. Molecular analyses identified the Fas death ligand (FasL) as a target for DkkL1 pro-apoptotic activity in adult mice. DKKL1 is considered as a negative regulator of adult testic homeostasis and identifies a novel, DKKL1 / FasL- dependent, regulation that specifically controls the number of postpubertal spermatocytes.