|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human GRK5 ORF mammalian expression plasmid, C-GFPSpark tag||HG10839-ACG|
|Human GRK5 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10839-ACR|
|Human GRK5 ORF mammalian expression plasmid, N-GFPSpark tag||HG10839-ANG|
|Human GRK5 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10839-ANR|
|Human GRK5 ORF mammalian expression plasmid, C-Flag tag||HG10839-CF|
|Human GRK5 ORF mammalian expression plasmid, C-His tag||HG10839-CH|
|Human GRK5 ORF mammalian expression plasmid, C-Myc tag||HG10839-CM|
|Human GRK5 ORF mammalian expression plasmid, C-HA tag||HG10839-CY|
|Human GRK5 Gene cDNA clone plasmid||HG10839-M|
|Human GRK5 ORF mammalian expression plasmid, N-Flag tag||HG10839-NF|
|Human GRK5 ORF mammalian expression plasmid, N-His tag||HG10839-NH|
|Human GRK5 ORF mammalian expression plasmid, N-Myc tag||HG10839-NM|
|Human GRK5 ORF mammalian expression plasmid, N-HA tag||HG10839-NY|
|Human GRK5 natural ORF mammalian expression plasmid||HG10839-UT|
|Learn more about expression Vectors|
G protein-coupled receptor kinase 5, also known as G protein-coupled receptor kinase GRK5 and GRK5, is a member of the protein kinase superfamily, AGC Ser/Thr protein kinase family and GPRK subfamily. GRKs specifically phosphorylate agonist-occupied G protein-coupled receptors at the inner surface of the plasma membrane (PM), leading to receptor desensitization. GRKs utilize a variety of mechanisms to bind tightly, and sometimes reversibly, to cellular membranes. GRKs play an important role in mediating agonist-specific desensitization of numerous G protein-coupled receptors.
GRK5 contains one AGC-kinase C-terminal domain, one protein kinase domain and one RGS domain. GRK5 specifically phosphorylates the activated forms of G protein-coupled receptors. Phospholipid-stimulated autophosphorylation may represent a novel mechanism for membrane association and regulation of GRK5 activity. GRK5 deficiency significantly exaggerates microgliosis and astrogliosis in the presence of an inflammatory initiator, such as the excess fibrillar Abeta and the subsequent active inflammatory reactions. GRK5 deficiency has been linked to early Alzheimer's disease in humans and mouse models of the disease.