|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human PNLIPRP1 ORF mammalian expression plasmid, C-GFPSpark tag||HG13565-ACG|
|Human PNLIPRP1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13565-ACR|
|Human PNLIPRP1 ORF mammalian expression plasmid, C-Flag tag||HG13565-CF|
|Human PNLIPRP1 ORF mammalian expression plasmid, C-His tag||HG13565-CH|
|Human PNLIPRP1 ORF mammalian expression plasmid, C-Myc tag||HG13565-CM|
|Human PNLIPRP1 ORF mammalian expression plasmid, C-HA tag||HG13565-CY|
|Human PNLIPRP1 Gene cDNA clone plasmid||HG13565-G|
|Human PNLIPRP1 ORF mammalian expression plasmid, N-Flag tag||HG13565-NF|
|Human PNLIPRP1 ORF mammalian expression plasmid, N-His tag||HG13565-NH|
|Human PNLIPRP1 ORF mammalian expression plasmid, N-Myc tag||HG13565-NM|
|Human PNLIPRP1 ORF mammalian expression plasmid, N-HA tag||HG13565-NY|
|Human PNLIPRP1 natural ORF mammalian expression plasmid||HG13565-UT|
|Learn more about expression Vectors|
PNLIPRP1, also known as PLRP1, belongs to the AB hydrolase superfamily, Lipase family. PNLIPRP1 is structurally similar to PLRP2. However, these two proteins display different functional properties. PNLIPRP1 may function as inhibitor of dietary triglyceride digestion. It lacks detectable lipase activity towards triglycerides, diglycerides, phosphatidylcholine, galactolipids or cholesterol esters. PLRP2 hydrolyses milk fat with a lower catalytic efficiency than that of PL. PLRP2 activity, higher on homogenized than on native milk fat, is differently influenced by fatty acids and colipase depending on a proteolytic cleavage in the lid domain.