|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human PNLIP ORF mammalian expression plasmid, C-GFPSpark tag||HG13564-ACG|
|Human PNLIP ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13564-ACR|
|Human PNLIP ORF mammalian expression plasmid, C-Flag tag||HG13564-CF|
|Human PNLIP ORF mammalian expression plasmid, C-His tag||HG13564-CH|
|Human PNLIP ORF mammalian expression plasmid, C-Myc tag||HG13564-CM|
|Human PNLIP ORF mammalian expression plasmid, C-HA tag||HG13564-CY|
|Human PNLIP Gene cDNA clone plasmid||HG13564-G|
|Human PNLIP ORF mammalian expression plasmid, N-Flag tag||HG13564-NF|
|Human PNLIP ORF mammalian expression plasmid, N-His tag||HG13564-NH|
|Human PNLIP ORF mammalian expression plasmid, N-Myc tag||HG13564-NM|
|Human PNLIP ORF mammalian expression plasmid, N-HA tag||HG13564-NY|
|Human PNLIP natural ORF mammalian expression plasmid||HG13564-UT|
|Learn more about expression Vectors|
PNLIP is an enzyme which belongs to the lipase family. Secreted from the pancreas, PNLIP is the primary lipase that hydrolyzes dietary fat molecules in the human digestive system, converting triglyceride substrates found in ingested oils to monoglycerides and free fatty acids. Bile salts secreted from the liver and stored in gallbladder are released into the duodenum where they coat and emulsify large fat droplets into smaller droplets, thus increasing the overall surface area of the fat, which allows the lipase to break apart the fat more effectively. The resulting monomers (2 free fatty acids and one 2-monoacylglycerol) are then moved by way of peristalsis along the small intestine to be absorbed into the lymphatic system by a specialized vessel called a lacteal.