|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human GIF ORF mammalian expression plasmid, C-GFPSpark tag||HG13544-ACG|
|Human GIF ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13544-ACR|
|Human GIF ORF mammalian expression plasmid, C-Flag tag||HG13544-CF|
|Human GIF ORF mammalian expression plasmid, C-His tag||HG13544-CH|
|Human GIF ORF mammalian expression plasmid, C-Myc tag||HG13544-CM|
|Human GIF ORF mammalian expression plasmid, C-HA tag||HG13544-CY|
|Human GIF Gene cDNA clone plasmid||HG13544-G|
|Human GIF ORF mammalian expression plasmid, N-Flag tag||HG13544-NF|
|Human GIF ORF mammalian expression plasmid, N-His tag||HG13544-NH|
|Human GIF ORF mammalian expression plasmid, N-Myc tag||HG13544-NM|
|Human GIF ORF mammalian expression plasmid, N-HA tag||HG13544-NY|
|Human GIF natural ORF mammalian expression plasmid||HG13544-UT|
|Learn more about expression Vectors|
Gastric intrinsic factor, also known as GIF, belongs to the of the cobalamin transport protein family. It is a glycoprotein produced by the parietal cells of the stomach. Gastric intrinsic factor plays a key role in the absorption of vitamin B12 on in the small intestine. Vitamin B12 bounds to haptocorrin after entry into the stomach. The resulting complex enters the duodenum, where pancreatic enzymes digest haptocorrin. In the less acidic environment of the small intestine, B12 can then bind to gastric intrinsic factor. This new complex travels to the ileum, where special epithelial cells endocytose them. Inside the cell, B12 dissociates once again and binds to another protein, transcobalamin II. The new complex can exit the epithelial cells to enter the liver.