|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human ART3 ORF mammalian expression plasmid, C-GFPSpark tag||HG13542-ACG|
|Human ART3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13542-ACR|
|Human ART3 ORF mammalian expression plasmid, C-Flag tag||HG13542-CF|
|Human ART3 ORF mammalian expression plasmid, C-His tag||HG13542-CH|
|Human ART3 ORF mammalian expression plasmid, C-Myc tag||HG13542-CM|
|Human ART3 ORF mammalian expression plasmid, C-HA tag||HG13542-CY|
|Human ART3 Gene cDNA clone plasmid||HG13542-G|
|Human ART3 ORF mammalian expression plasmid, N-Flag tag||HG13542-NF|
|Human ART3 ORF mammalian expression plasmid, N-His tag||HG13542-NH|
|Human ART3 ORF mammalian expression plasmid, N-Myc tag||HG13542-NM|
|Human ART3 ORF mammalian expression plasmid, N-HA tag||HG13542-NY|
|Human ART3 natural ORF mammalian expression plasmid||HG13542-UT|
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ART3 is an arginine-specific ADP-ribosyltransferase which belongs to the Arg-specific ADP-ribosyltransferase family. ART3 catalyzes a reversible reaction which modifies proteins by the addition or removal of ADP-ribose to an arginine residue to regulate the function of the modified protein. It is expressed specifically in testis. ART3 pseudogene is located on chromosome 11. ART3 was identified as a susceptibility gene for non-obstructive azoospermia (NOA). It is a novel therapeutic target in the treatment of NOA.