|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human BACE2 ORF mammalian expression plasmid, C-GFPSpark tag||HG10783-ACG|
|Human BACE2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10783-ACR|
|Human BACE2 ORF mammalian expression plasmid, C-Flag tag||HG10783-CF|
|Human BACE2 ORF mammalian expression plasmid, C-His tag||HG10783-CH|
|Human BACE2 ORF mammalian expression plasmid, C-Myc tag||HG10783-CM|
|Human BACE2 ORF mammalian expression plasmid, C-HA tag||HG10783-CY|
|Human BACE2 Gene cDNA clone plasmid||HG10783-M|
|Human BACE2 ORF mammalian expression plasmid, N-Flag tag||HG10783-NF|
|Human BACE2 ORF mammalian expression plasmid, N-His tag||HG10783-NH|
|Human BACE2 ORF mammalian expression plasmid, N-Myc tag||HG10783-NM|
|Human BACE2 ORF mammalian expression plasmid, N-HA tag||HG10783-NY|
|Human BACE2 natural ORF mammalian expression plasmid||HG10783-UT|
|Learn more about expression Vectors|
BACE2, also known as beta secretase 2, belongs to the peptidase A1 family. It is a protease known to be an important enzyme involved in the cellular pathways. BACE2 has been shown to interact with GGA1 and GGA2. It is the major β-secretase in vivo. BACE2 is located on chromosome 21 and may play a role in alzheimer's disease pathogenesis in down syndrome(DS). Overexpression of BACE2 by lentivirus markedly reduced amyloid β protein production in primary neurons. Despite an extra copy of the BACE2 gene in DS and the increase of its transcription, BACE2 protein levels are unchanged.