|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human DCX ORF mammalian expression plasmid, C-GFPSpark tag||HG13498-ACG|
|Human DCX ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13498-ACR|
|Human DCX ORF mammalian expression plasmid, N-GFPSpark tag||HG13498-ANG|
|Human DCX ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG13498-ANR|
|Human DCX ORF mammalian expression plasmid, C-Flag tag||HG13498-CF|
|Human DCX ORF mammalian expression plasmid, C-His tag||HG13498-CH|
|Human DCX ORF mammalian expression plasmid, C-Myc tag||HG13498-CM|
|Human DCX ORF mammalian expression plasmid, C-HA tag||HG13498-CY|
|Human DCX Gene cDNA clone plasmid||HG13498-G|
|Human DCX ORF mammalian expression plasmid, N-Flag tag||HG13498-NF|
|Human DCX ORF mammalian expression plasmid, N-His tag||HG13498-NH|
|Human DCX ORF mammalian expression plasmid, N-Myc tag||HG13498-NM|
|Human DCX ORF mammalian expression plasmid, N-HA tag||HG13498-NY|
|Human DCX natural ORF mammalian expression plasmid||HG13498-UT|
|Learn more about expression Vectors|
DCX (doublecortin, N-GST chimera)contains 2 doublecortin domains and belongs to the doublecortin family. It is highly expressed in neuronal cells of fetal brain, but not expressed in other fetal tissues. In the adult, it is highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas. DCX is a microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. It may act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. DCX may in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. It may be part with LIS-1 of a overlapping, but distinct, signaling pathways that promote neuronal migration. Defects in DCX are the cause of lissencephaly X-linked type 1 and subcortical band heterotopia X-linked.