|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human SUSD4 ORF mammalian expression plasmid, C-GFPSpark tag||HG13488-ACG|
|Human SUSD4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13488-ACR|
|Human SUSD4 ORF mammalian expression plasmid, C-Flag tag||HG13488-CF|
|Human SUSD4 ORF mammalian expression plasmid, C-His tag||HG13488-CH|
|Human SUSD4 ORF mammalian expression plasmid, C-Myc tag||HG13488-CM|
|Human SUSD4 ORF mammalian expression plasmid, C-HA tag||HG13488-CY|
|Human SUSD4 Gene cDNA clone plasmid||HG13488-G|
|Human SUSD4 ORF mammalian expression plasmid, N-Flag tag||HG13488-NF|
|Human SUSD4 ORF mammalian expression plasmid, N-His tag||HG13488-NH|
|Human SUSD4 ORF mammalian expression plasmid, N-Myc tag||HG13488-NM|
|Human SUSD4 ORF mammalian expression plasmid, N-HA tag||HG13488-NY|
|Human SUSD4 natural ORF mammalian expression plasmid||HG13488-UT|
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SUSD4, also known as sushi domain-containing protein 4, is a hypothetical cell surface protein whose tissue distribution and function are completely unknown. SUSD4 is detectable in murine brains, eyes, spinal cords, and testis but not other tissues. In brains, SUSD4 is highly expressed in the white matter on oligodendrocytes/axons, and in eyes, it is exclusively expressed on the photoreceptor outer segments. In in vitro complement assays, SUSD4 augments the alternative but not the classical pathway of complement activation at the C3 convertase step. SUSD4 deficiency may cause autism or Fryns syndrome, both of which are genetic diseases with severe abnormal neurological development and/or functions.