|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human SEMA3A ORF mammalian expression plasmid, C-GFPSpark tag||HG10758-ACG|
|Human SEMA3A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10758-ACR|
|Human SEMA3A ORF mammalian expression plasmid, C-Flag tag||HG10758-CF|
|Human SEMA3A ORF mammalian expression plasmid, C-His tag||HG10758-CH|
|Human SEMA3A ORF mammalian expression plasmid, C-Myc tag||HG10758-CM|
|Human SEMA3A ORF mammalian expression plasmid, C-HA tag||HG10758-CY|
|Human SEMA3A natural ORF mammalian expression plasmid||HG10758-M-N|
|Human SEMA3A ORF mammalian expression plasmid, N-Flag tag||HG10758-NF|
|Human SEMA3A ORF mammalian expression plasmid, N-His tag||HG10758-NH|
|Human SEMA3A ORF mammalian expression plasmid, N-Myc tag||HG10758-NM|
|Human SEMA3A ORF mammalian expression plasmid, N-HA tag||HG10758-NY|
|Human SEMA3A natural ORF mammalian expression plasmid||HG10758-UT|
|Learn more about expression Vectors|
Semaphorins are a family of secreted and cell-bound signaling molecules defined by the presence of a common 500 aa Sema domain. They are best characterized in relation to axon guidance during development of the nervous system. The functions of Semaphorins 3A (SEMA3A) are mediated primarily through binding to the Neuropilin-1 (Npn-1) and Plexin-A1 coreceptor complex. Neuropilins lack a signaling-competent cytoplasetmic domain and ensure semaphorin binding, whereas the transmembrane receptor plexin mediates the intracellular response. As the first identified vertebrate semaphorin, SEMA3A functions either as a chemorepulsive agent inhibiting axonal outgrowth, or as a chemoattractive agent stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Its overexpression is associated with schizophrenia which is seen in various human tumor cell lines, and aberrant release is associated with the progression of Alzheimer's disease