|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human C10ORF54 ORF mammalian expression plasmid, C-GFPSpark tag||HG13482-ACG|
|Human C10ORF54 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13482-ACR|
|Human C10ORF54 ORF mammalian expression plasmid, C-Flag tag||HG13482-CF|
|Human C10ORF54 ORF mammalian expression plasmid, C-His tag||HG13482-CH|
|Human C10ORF54 ORF mammalian expression plasmid, C-Myc tag||HG13482-CM|
|Human C10ORF54 ORF mammalian expression plasmid, C-HA tag||HG13482-CY|
|Human C10ORF54 Gene cDNA clone plasmid||HG13482-G|
|Human C10ORF54 ORF mammalian expression plasmid, N-Flag tag||HG13482-NF|
|Human C10ORF54 ORF mammalian expression plasmid, N-His tag||HG13482-NH|
|Human C10ORF54 ORF mammalian expression plasmid, N-Myc tag||HG13482-NM|
|Human C10ORF54 ORF mammalian expression plasmid, N-HA tag||HG13482-NY|
|Human C10ORF54 natural ORF mammalian expression plasmid||HG13482-UT|
|Learn more about expression Vectors|
C10orf54, also known as GI24, belongs to the immunoglobulin superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESCs, C10orf54 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, C10orf54 both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Human C10orf54 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment.