|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human DPP-II ORF mammalian expression plasmid, C-GFPSpark tag||HG10748-ACG|
|Human DPP-II ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10748-ACR|
|Human DPP-II ORF mammalian expression plasmid, C-Flag tag||HG10748-CF|
|Human DPP-II ORF mammalian expression plasmid, C-His tag||HG10748-CH|
|Human DPP-II ORF mammalian expression plasmid, C-Myc tag||HG10748-CM|
|Human DPP-II ORF mammalian expression plasmid, C-HA tag||HG10748-CY|
|Human DPP-II Gene cDNA clone plasmid||HG10748-M|
|Human DPP-II ORF mammalian expression plasmid, N-Flag tag||HG10748-NF|
|Human DPP-II ORF mammalian expression plasmid, N-His tag||HG10748-NH|
|Human DPP-II ORF mammalian expression plasmid, N-Myc tag||HG10748-NM|
|Human DPP-II ORF mammalian expression plasmid, N-HA tag||HG10748-NY|
|Human DPP-II natural ORF mammalian expression plasmid||HG10748-UT|
|Learn more about expression Vectors|
DPP7 (dipeptidylpeptidase 7), also known as DPPII and DPP2, is a post-proline cleaving aminopeptidase expressed in quiescent lymphocytes. Dipeptidyl peptidases (DPPs) have post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. DPPs mediate regulatory activity of their substrates and have been linked to a variety of diseases including type 2 diabetes, obesity and cancer. DPPs can bind specific voltage-gated potassium channels and alter their expression and biophysical properties and may also influence T cells. DPP proteins include DPRP1, DPRP2, DPP3, DPP7, DPP10, DPPX and CD26. It localizes to lysosomes. DPP7 localizes to lysosomes and exists as a homodimer via its leucine zipper motif and is involved in the degradation of oligopeptides. In response to calcium release, it can be secreted in its active form. It is essential for lymphocyte survival, as the inhibition of DPP7 results in quiescent cell apoptosis.