|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human PRSS7 ORF mammalian expression plasmid, C-GFPSpark tag||HG10733-ACG|
|Human PRSS7 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10733-ACR|
|Human PRSS7 ORF mammalian expression plasmid, C-Flag tag||HG10733-CF|
|Human PRSS7 ORF mammalian expression plasmid, C-His tag||HG10733-CH|
|Human PRSS7 ORF mammalian expression plasmid, C-Myc tag||HG10733-CM|
|Human PRSS7 ORF mammalian expression plasmid, C-HA tag||HG10733-CY|
|Human PRSS7 Gene cDNA clone plasmid||HG10733-M|
|Human PRSS7 ORF mammalian expression plasmid, N-Flag tag||HG10733-NF|
|Human PRSS7 ORF mammalian expression plasmid, N-His tag||HG10733-NH|
|Human PRSS7 ORF mammalian expression plasmid, N-Myc tag||HG10733-NM|
|Human PRSS7 ORF mammalian expression plasmid, N-HA tag||HG10733-NY|
|Human PRSS7 natural ORF mammalian expression plasmid||HG10733-UT|
|Learn more about expression Vectors|
Enterokinase, or Enteropeptidase is a type II transmembrane, which is a member of the trypsin family of serine proteases, and plays a key role in mammalian metabolism. It is synthesized as a zymogen (proenteropeptidase) that requires activation by another protease, either trypsin or possibly duodenase. Active enteropeptidase then converts the pancreatic precursor, trypsinogen, to trypsin by cleavage of the specific trypsinogen activation peptide, Asp-Asp-Asp-Asp-Lys- Ile that is highly conserved in vertebrates. The mature trypsin in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases. Enterokinase consists of two subunits linked by a disulfide bond. The heavy chain achors enterokinase in the intestinal brush border membrane and the light chain is the catalytic subunit, which has the same mechanism of action as trypsin and chymotrypsin. Enterokinase is the physiological activator of trypsinogen and has a specificity for the sequence (Asp)4-Lys-Ile. Because of its high specificity towards the amino acid sequence (Asp)(4)-Lys, enterokinase is a potential tool for the cleavage of fusion proteins, which are gaining more importance in biopharmaceutical production. In addition, Enterokinase is a tool protease widely utilized in the cleavage of recombinant fusion proteins.