|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CLK3 ORF mammalian expression plasmid, C-GFPSpark tag||HG10716-ACG|
|Human CLK3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10716-ACR|
|Human CLK3 ORF mammalian expression plasmid, N-GFPSpark tag||HG10716-ANG|
|Human CLK3 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10716-ANR|
|Human CLK3 ORF mammalian expression plasmid, C-Flag tag||HG10716-CF|
|Human CLK3 ORF mammalian expression plasmid, C-His tag||HG10716-CH|
|Human CLK3 ORF mammalian expression plasmid, C-Myc tag||HG10716-CM|
|Human CLK3 ORF mammalian expression plasmid, C-HA tag||HG10716-CY|
|Human CLK3 ORF mammalian expression plasmid, N-Flag tag||HG10716-NF|
|Human CLK3 ORF mammalian expression plasmid, N-His tag||HG10716-NH|
|Human CLK3 ORF mammalian expression plasmid, N-Myc tag||HG10716-NM|
|Human CLK3 ORF mammalian expression plasmid, N-HA tag||HG10716-NY|
|Human CLK3 Gene cDNA clone plasmid||HG10716-U|
|Human CLK3 natural ORF mammalian expression plasmid||HG10716-UT|
|Learn more about expression Vectors|
Dual specificity protein kinase CLK3, also known as CDC-like kinase 3, and CLK3, is a member of CMGC Ser/Thr protein kinase family and Lammer subfamily. Mammalian CLK is the prototype for a family of dual specificity kinases (termed Lammer kinases) that have been conserved in evolution. CLK family members have shown to interact with, and phosphorylate, serine- and arginine-rich (SR) proteins of the spliceosomal complex, which is a part of the regulatory mechanism that enables the SR proteins to control RNA splicing. The three members of the CLK family of kinases (CLK1, CLK2, and CLK3) have been shown to undergo conserved alternative splicing to generate catalytically active and inactive isoforms. The human CLK2 and CLK3 are found within the nucleus and display dual-specificity kinase activity. The truncated isoforms, hCLK2(T) and hCLK3(T), colocalize with SR proteins in nuclear speckles. CLK3 may play a role in the development and progression of azoospermia.