|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human METRN ORF mammalian expression plasmid, C-GFPSpark tag||HG13433-ACG|
|Human METRN ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13433-ACR|
|Human METRN ORF mammalian expression plasmid, C-Flag tag||HG13433-CF|
|Human METRN ORF mammalian expression plasmid, C-His tag||HG13433-CH|
|Human METRN ORF mammalian expression plasmid, C-Myc tag||HG13433-CM|
|Human METRN ORF mammalian expression plasmid, C-HA tag||HG13433-CY|
|Human METRN Gene cDNA clone plasmid||HG13433-G|
|Human METRN ORF mammalian expression plasmid, N-Flag tag||HG13433-NF|
|Human METRN ORF mammalian expression plasmid, N-His tag||HG13433-NH|
|Human METRN ORF mammalian expression plasmid, N-Myc tag||HG13433-NM|
|Human METRN ORF mammalian expression plasmid, N-HA tag||HG13433-NY|
|Human METRN natural ORF mammalian expression plasmid||HG13433-UT|
|Learn more about expression Vectors|
Meteorin is a novel secreted protein that is expressed in undifferentiated neural progenitors and in the astrocyte lineage, including radial glia. It plays important roles in the differentiation of glial cells and also in axonal network formation during neurogenesis. Meteorin selectively promoted astrocyte formation from mouse cerebrocortical neurospheres in differentiation culture, whereas it induced cerebellar astrocytes to become radial glia. Meteorin also induced axonal extension in small and intermediate neurons of sensory ganglia by activating nearby satellite glia.