Quick Order

Human EpCAM / TROP1 Gene cDNA Clone (full-length ORF Clone), expression ready, N-FLAG-tagged

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
EPCAMcDNA Clone Product Information
Gene_bank_ref_id:NM_002354.2
cDNA Size:963
cDNA Description:ORF Clone of Homo sapiens tumor-associated calcium signal transducer 1 DNA.
Gene Synonym:TACSTD1, EGP, KSA, M4S1, MK-1, CD326, EGP40, MIC18, TROP1, Ep-CAM, hEGP-2, CO17-1A, GA733-2??
Species:Human
Vector:pCMV3-SP-N-FLAG
Restriction Site:KpnI + XbaI
Tag Sequence:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequence Description:Identical with the Gene Bank Ref. ID sequence.
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pCMV3-SP-N-FLAG (suitable for secretary and membane protein expession) Vector Information
 
Vector Name pCMV3-SP-N-FLAG
Vector Size 6143bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive, Stable / Transient
Promoter CMV
Antibiotic Resistance Kanamycin
Selection In Mammalian Cells Hygromycin
Protein Tag FLAG
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)

pCMV3-SP-N-FLAG (suitable for secretary and membane protein expession) Physical Map
Schematic of pCMV3-SP-N-FLAG (suitable for secretary and membane protein expession) Multiple Cloning Sites

FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Human EpCAM / TROP1 Gene cDNA Clone (full-length ORF Clone), expression ready, N-FLAG-tagged on other vectors
Human EpCAM / TROP1 Gene cDNA Clone (full-length ORF Clone), expression ready, C-GFPSpark-taggedHG10694-ACG$325
Human EpCAM / TROP1 Gene cDNA Clone (full-length ORF Clone), expression ready, C-OFPSpark tagHG10694-ACR$325
Human EpCAM / TROP1 Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-taggedHG10694-CF$295
Human EpCAM / TROP1 Gene cDNA Clone (full-length ORF Clone), expression ready, C-His-taggedHG10694-CH$295
Human EpCAM / TROP1 Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-taggedHG10694-CM$295
Human EpCAM / TROP1 Gene cDNA Clone (full-length ORF Clone), expression ready, C-HA-taggedHG10694-CY$295
Human EPCAM Gene cDNA Clone (full-length ORF Clone)HG10694-G$95
Human EpCAM / TROP1 Gene cDNA Clone (full-length ORF Clone), expression ready, His-taggedHG10694-G-H$295
Human EpCAM / TROP1 Gene cDNA Clone (full-length ORF Clone) expression ready, Myc-taggedHG10694-G-M$295
Human EpCAM / TROP1 Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG10694-G-N$295
Human EpCAM / TROP1 Gene cDNA Clone (full-length ORF Clone), expression ready, N-FLAG-taggedHG10694-NF$295
Human EpCAM / TROP1 Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-taggedHG10694-NH$295
Human EpCAM / TROP1 Gene cDNA Clone (full-length ORF Clone), expression ready, N-Myc-taggedHG10694-NM$295
Human EpCAM / TROP1 Gene cDNA Clone (full-length ORF Clone), expression ready, N-HA-taggedHG10694-NY$295
Human EpCAM / TROP1 Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG10694-UT$295
 Learn more about expression Vectors
Related Products
Product nameProduct name
Background

Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type â… transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.

References
  • Brunner A, et al. (2008) EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder. J Clin Pathol. 61(3):307-10.
  • Trzpis M, et al. (2008) EpCAM in morphogenesis. Front Biosci. 13: 5050-5.
  • Munz M, et al. (2009) The emerging role of EpCAM in cancer and stem cell signaling. Cancer Res. 69(14): 5627-9.
  • Carpenter G, et al. (2009) EpCAM: another surface-to-nucleus missile. Cancer Cell. 15(3): 165-6.
  • Size / Price
    Catalog:HG10694-NF
    List Price: $295.00  (Save $0.00)
    Price:$295.00      [How to order]
    Availability5 business days
    Images
    • Human EpCAM / TROP1 Gene cDNA Clone (full-length ORF Clone), expression ready, N-FLAG-tagged
    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"