|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human ULBP-1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10679-ACG|
|Human ULBP-1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10679-ACR|
|Human ULBP-1 ORF mammalian expression plasmid, C-Flag tag||HG10679-CF|
|Human ULBP-1 ORF mammalian expression plasmid, C-His tag||HG10679-CH|
|Human ULBP-1 ORF mammalian expression plasmid, C-Myc tag||HG10679-CM|
|Human ULBP-1 ORF mammalian expression plasmid, C-HA tag||HG10679-CY|
|Human ULBP-1 Gene cDNA clone plasmid||HG10679-M|
|Human ULBP-1 ORF mammalian expression plasmid, Flag tag||HG10679-M-F|
|Human ULBP-1 ORF mammalian expression plasmid, N-Flag tag||HG10679-NF|
|Human ULBP-1 ORF mammalian expression plasmid, N-His tag||HG10679-NH|
|Human ULBP-1 ORF mammalian expression plasmid, N-Myc tag||HG10679-NM|
|Human ULBP-1 ORF mammalian expression plasmid, N-HA tag||HG10679-NY|
|Human ULBP-1 natural ORF mammalian expression plasmid||HG10679-UT|
|Learn more about expression Vectors|
UL16-binding proteins (ULBP) or retinoic acid early transcripts-1 (RAET1) are ligands to the activating receptor, NKG2D. Ten members of the human ULBP/RAET1 gene family have been identified to encode for potentially functional proteins, and have tissue-specific expressions. ULBP1, also known as RAET1I and NKG2DL1, together with at least ULBP 2 and 3, are well-known ligands for NKG2D, and activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. ULBP1 is expressed in T-cells, B-cells, erythroleukemia cell lines and in a wide range of tissues including heart, brain, lung, liver and bone marrow, as well as some tumor cells. As an unconventional member of the MHC class I family, ULBP1 function in immune responses, especially in cancer and infectious diseases. Unlike other ULBP members, ULBP1 is able to interact with soluble CMV glycoprotein UL16 in CMV infected cells. The interaction with UL16 blocked the interaction with the NKG2D receptor, and thus might escape the immune surveillance. Furthermore, UL16 also causes ULBP1 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface. The ULBP1 regulation may have implications for development of new therapeutic strategies against cancer cells.
1. Rölle, A. et al., 2003, J Immunol. 171(2): 902-908.
2. López-Soto, A. et al., 2006, J Biol Chem. 281(41): 30419-30430.
3. Song, H. et al., 2006, Cell Immunol. 239(1): 22-30.
4. Eisele, G. et al., 2006, Brain. 129 (9): 2416-2425.
5. Romphruk, AV. et al., 2009, Immunogenetics. 61(9): 611-617.
6. Sutherland, C.L. et. al., 2002, J. Immunol. 168: 671-679.