|Datasheet||Specific References||Reviews||Related Products||Protocols|
|RP23-295E4.1, 9030024J15Rik, AI552599, Erbb, Errp, Wa5, wa-2, wa|
|A DNA sequence encoding the extracellular domain of mouse EGFR (Q01279) (Met 1-Ser 647) was fused with a polyhistidine tag at the C-terminus.|
|In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.|
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
|> 95 % as determined by SDS-PAGE|
|1. Measured by its binding ability in a functional ELISA.|
2. Immobilized mouse EGFR-his at 10 μg/mL (100 μl/well) can bind human EGF-Fc (Cat:10605-H01H), The EC50 of human EGF-Fc (Cat:10605-H01H) is 60-90 ng/mL.
3. Immobilized mouse EGFR-his at 10 μg/mL (100 μl/well) can bind mouse EGF-Fc (Cat:50482-M01H), The EC50 of mouse EGF-Fc (Cat:50482-M01H) is 70-100 ng/mL.
|< 1.0 EU per μg of the protein as determined by the LAL method|
|Samples are stable for up to twelve months from date of receipt at -70℃|
|The secreted recombinant mouse EGFR comprises 634 amino acids and has a calculated molecular mass of 71 kDa. As a result of glycosylation, the apparent molecular mass of rmEGFR is approximately 100 kDa in SDS-PAGE under reducing conditions.|
|Lyophilized from sterile PBS, pH 7.4|
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
|Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|
As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.