|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human RP2 ORF mammalian expression plasmid, C-GFPSpark tag||HG10637-ACG|
|Human RP2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10637-ACR|
|Human RP2 ORF mammalian expression plasmid, C-Flag tag||HG10637-CF|
|Human RP2 ORF mammalian expression plasmid, C-His tag||HG10637-CH|
|Human RP2 ORF mammalian expression plasmid, C-Myc tag||HG10637-CM|
|Human RP2 ORF mammalian expression plasmid, C-HA tag||HG10637-CY|
|Human RP2 Gene cDNA clone plasmid||HG10637-M|
|Human RP2 ORF mammalian expression plasmid, N-Flag tag||HG10637-NF|
|Human RP2 ORF mammalian expression plasmid, N-His tag||HG10637-NH|
|Human RP2 ORF mammalian expression plasmid, N-Myc tag||HG10637-NM|
|Human RP2 ORF mammalian expression plasmid, N-HA tag||HG10637-NY|
|Human RP2 natural ORF mammalian expression plasmid||HG10637-UT|
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XRP2, also known as Protein XRP2 and RP2, is a member of the TBCC (tubulin cofactor C) family and contains one C-CAP/cofactor C-like domain. This protein is encoded by the RP2 gene in humans. XRP2 stimulates the GTPase activity of tubulin, but does not enhance tubulin heterodimerization. XRP2 acts as guanine nucleotide dissociation inhibitor for ARL3. Defects in RP2 gene are the cause of retinitis pigmentosa type 2 (RP2), also known as X-linked retinitis pigmentosa 2 (XLRP-2). It leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.