|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human GREM1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10632-ACG|
|Human GREM1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10632-ACR|
|Human GREM1 ORF mammalian expression plasmid, C-Flag tag||HG10632-CF|
|Human GREM1 ORF mammalian expression plasmid, C-His tag||HG10632-CH|
|Human GREM1 ORF mammalian expression plasmid, C-Myc tag||HG10632-CM|
|Human GREM1 ORF mammalian expression plasmid, C-HA tag||HG10632-CY|
|Human GREM1 Gene cDNA clone plasmid||HG10632-G|
|Human GREM1 ORF mammalian expression plasmid, N-Flag tag||HG10632-NF|
|Human GREM1 ORF mammalian expression plasmid, N-His tag||HG10632-NH|
|Human GREM1 ORF mammalian expression plasmid, N-Myc tag||HG10632-NM|
|Human GREM1 ORF mammalian expression plasmid, N-HA tag||HG10632-NY|
|Human GREM1 natural ORF mammalian expression plasmid||HG10632-UT|
|Learn more about expression Vectors|
GREM1 belongs to the DAN family. It contains 1 CTCK (C-terminal cystine knot-like) domain. GREM1 is a cysteine knot-secreted protein and acts as an inhibitor in the TGF beta signaling pathway. It inhibits BMP-2, -4, and -7. Inhibition by grem 1 of BMPs in mice allow the expression of fibroblast growth factors (FGFs) 4 and 8 and Sonic hedgehog (SHH) which are necessary for proper limb development. It interacts with SLIT1 and SLIT2 in a glycosylation-dependent manner. As a cytokine, GREM1 may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. It down-regulates the BMP4 signaling in a dose-dependent manner. It also acts as inhibitor of monocyte chemotaxis. GREM1 is highly expressed in small intestine, fetal brain and colon.