|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CCL27 ORF mammalian expression plasmid, C-GFPSpark tag||HG10625-ACG|
|Human CCL27 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10625-ACR|
|Human CCL27 ORF mammalian expression plasmid, C-Flag tag||HG10625-CF|
|Human CCL27 ORF mammalian expression plasmid, C-His tag||HG10625-CH|
|Human CCL27 ORF mammalian expression plasmid, C-Myc tag||HG10625-CM|
|Human CCL27 ORF mammalian expression plasmid, C-HA tag||HG10625-CY|
|Human CCL27 Gene cDNA clone plasmid||HG10625-M|
|Human CCL27 ORF mammalian expression plasmid, Flag tag||HG10625-M-F|
|Human CCL27 ORF mammalian expression plasmid, N-Flag tag||HG10625-NF|
|Human CCL27 ORF mammalian expression plasmid, N-His tag||HG10625-NH|
|Human CCL27 ORF mammalian expression plasmid, N-Myc tag||HG10625-NM|
|Human CCL27 ORF mammalian expression plasmid, N-HA tag||HG10625-NY|
|Human CCL27 natural ORF mammalian expression plasmid||HG10625-UT|
|Learn more about expression Vectors|
CCL27, also known as CTACK, is a small cytokine belonging to the CC chemokine family. Members of this family are proteins characterized by two adjacent cysteines. CCL27 is chemotactic for skin-associated memory T lymphocytes. CCL27 may also play a role in mediating homing of lymphocytes to cutaneous sites. CCL27 plays a pivotal role in establishing the inflammatory infiltrate characteristic for common inflammatory skin diseases. Through binding to the chemokine receptor 10 (CCR10), CCL27 mediates inflammation by promoting lymphocyte migration into the skin.